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All constructs of robo (including wild type, full-length modifications and mini-robo modifications) used for transfection in COS cells were prepared in the pUB6/V5-HisA vector (Invitrogen) and all carried three copies of the HA tag in their N-terminal portion and a copy of the V5 tag followed by an His-Tag in the C-terminal portion.
ChIP was performed essentially as described (Stock et al., 2007) with some modifications used for specific antibodies, as detailed in the Extended Experimental Procedures.
Palmitoylation, which is the thioester linkage of palmitate to selected cysteine residues, is one of several lipid modifications used for tethering proteins to membranes [ 64].
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Future studies including measurement of gene-specific histone modifications using, for example, chromatin immunoprecipitation (ChIP) experiments would help to clarify this point.
The mechanism of surface modification used for RAs by accelerated carbonation is described below.
The mechanisms of surface modification used for RAs by acid treatments are described below.
The PICO TAG method, with minor modifications, was used for measuring the amino acid profile of the hydrolysate [12].
The real time RT-PCR assay that we have developed [15], [54] with minor modifications was used for the quantification of HCV RNA.
Appropriate modifications were used for searches in EMBASE and PsycINFO.
Similar statements with appropriate modifications were used for the second questionnaire part on perception of norm.
Repeat time stamp random case study following process modifications were used for comparison.
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