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However, these variants are much like protein modifications, the sample space of which is difficult to estimate explicitly.
Amylase activity in the supernatant was measured directly using the Phadebas kit (Magle Life Sciences, Lund, Sweden) according to the manufacturer's procedure, with the following modifications: the sample volume was 0.5 or 0.05 ml and the reactions were conducted at 50°C.
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This was used for all the material characterizations and for all the fluoride adsorption experiments without any surface modifications to the sample.
Realizing the need to improve our measurement setup, several modifications to the sample box shown in Figure8a were made in order to extend the detection limit of our device.
Since the T lymphocytes are significantly smaller in size (10 15 µm), modifications in the sample handling, for example, the use of micromanipulators, and reduction in the laser spot size are required.
This series of runs (13 in total) also included minor modifications to the sample preparation (e.g. protease digestion conditions and length of LC separation) and several biological and technical replicates.
Fourth, modifications to the sample inlet and pressure source had to be made to facilitate sample loading and minimize sample loss in the inlet.
We extracted total RNA using the mirVana miRNA isolation kit (Life Technologies) following the manufacturer's protocol with minor modifications to the sample homogenization stage.
Human total Aβ1 40 or Aβ1 42 content was measured by sandwich ELISA (#EZBRAIN40 and #EZBRAIN42, respectively; Millipore Corporation, Billerica, MA, USA) according to the manufacturer's instructions, with minor modifications in the sample preparation step.
After modification, the samples were extensively rinsed with absolute ethanol and followed by 500 °C annealing treatment for 1 h in air.
In order to increase the density of the surface hydroxyl groups before the actual surface modification, the samples were first submerged in deionized H2O at 100°C for 1 min. Subsequently, the samples were dried at 50°C to removethe liquid water from the surfaces.
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