Sentence examples for modifications the binding from inspiring English sources

EMSA was performed as previously described (Cohn et al., 2001) with the following modifications: the binding reaction that contained 1 μg of UHRF1 and 1 nM of radiolabeled DNA was performed in 10 μl of a solution containing 14 mM Tris-HCl (pH 8.0), 100 mM NaCl, 3.4% glycerol, 1 mM DTT, 20 ng poly dI·dC -poly dI·dC), and 1 μg BSA (New England BioLabs).

However, despite this slight structural modification, the binding mode of 3 differs dramatically from that of the first inhibitor, but provokes less pronounced induced-fit adaptations of the binding cavity.

However, modifications in the binding loop to achieve specificity may compromise structural rigidity and prevent re-engineered inhibitors from reaching optimal binding affinity.

How these modifications affect the binding of IL-3 to its receptor is not clear since the AA' loop does not make direct contact to either subunits of the receptor in the modeled structure (Figure 3A and 3C).

13a, 16 Unfortunately, the introduction of side chain modifications reduced the binding affinity of the ligands to integrins.

There are three main mechanisms in epigenetics: DNA methylation, histone modifications, and the binding of non-histone proteins [ 1].

Thus, the antifibrotic phenotype observed in fibroblasts submitted to proteasome inhibition results from profound modifications in the binding of key transcription factors.

Our findings indicate that both histone modifications and the binding of transcription factors could explain nearly 58% variation of the "openness" of chromatin structure.

These marks, often referred to as 'epigenetic marks' in the literature, include DNA methylation, histone variants, covalent histone modifications and the binding of chromatin-associated proteins, among others.

It is generally accepted that histone modifications and the binding of transcription factors are two main effectors for the "openness" of chromatin.

It is known that chromatin features such as histone modifications and the binding of transcription factors exert a significant impact on the "openness" of chromatin.