Beyond question, our results represent the modification, supplement, extension, and development of the above Theorem I.
Our result represents the modification, supplement, extension and improvement of some corresponding results in the references.
Our result represents the modification, supplement, extension and improvement of the above Theorem 1.1 in the following aspects.
AR42J cells were cultured in F-12K Nutrient Mixture (Kaighn's Modification), supplemented with 10%% (v/v) fetal bovine serum, 100 U/mL penicillin, 100 μg/mL streptomycin, and 1 mM L glutamine.
Cell mixtures were cultured in round-bottomed 96-well plates (Cellstar) in a total volume of 200 µl T cell media (RPMI 1640 with Hepes modification supplemented with L-Glutamine, Penicillin-Streptomycin and 10% Human AB Serum, Lot number 027K0432).
Cells were grown in RPMI 1640 (Dutch modification, supplemented with 10% v/v foetal bovine serum, and antibiotics (Gibco BRL)) at 37°C/5% CO2.
Cells were washed twice with PBS and resuspended in RPMI-1640+ RPMI-1640+ RPMI-1640 RPMI-1640supplementeDutchh 10 μg/modificationn, 10 mM L-glutamine, and 10 mM pyruvate) (Gibco, Invitrogen, Breda, The Netherlandsupplemented
Osteoblasts were isolated from the calvaria of newborn Hif-1α-floxed, Hif-2α-floxed, mTOR-floxed and Vhl-floxed mice by serial digestion in 1.8 mg·mL−1 collagenase type I and maintained in α-MEM (minimum essential medium, alpha modification) supplemented with 10% FBS (Fetal bovine serum) and 1% penicillin/streptomycin.
The aortic arch was attached to a Langendorff apparatus, and retrogradely perfused with calcium-free perfusion buffer containing minimum essential medium (Joklik's modification) supplemented with 5 mM taurine, 2 mM creatine, 5 mM HEPES and 20 u/l insulin for 5 minutes.
A variety of dietary modifications and supplements have been tried with only moderate success in prevention of the disease.
