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Total RNA from minimal cell numbers was also extracted using the RNeasy Micro RNA isolation kit with the following modifications (process B) (Figure 1A).
When isolating RNA from titrated minimal cell numbers (3000 – 200) total RNA was isolated using the RNeasy Micro RNA isolation kit (Qiagen) following the manufacturers instructions (process A) or with modifications (process B) (Figure 1A).
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"Our duty is to have a fair modification process".
The current loan modification process is plagued by bureaucratic runarounds.
To use LoanPort, borrowers must engage a free Hope Now housing counselor to help lead them through the modification process.
A year later, she and Roman had finally tunneled through the interminable loan-modification process.
City officials have sometimes used the budget-modification process to push through significant changes.
He said the modification process had gone on for several years.
All the banks also deceived troubled borrowers, the complaint said, about the loan modification process.
Widespread reports of such problems help to underscore the wisdom of using a free housing counselor in the loan-modification process, Ms. Schwartz said.
"We have continued to improve our modification process to help 404,000 families get permanent modifications," he said.
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