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In particular, using subtle coding together with the modifications outlined in the last few paragraphs gives us networks that satisfy the conclusion of Theorem 7.
RNA was extracted using an RNeasy® Mini Kit (Qiagen Inc., Valencia, CA) with modifications outlined in Brown et al. [ 44] for extraction with glass beads using bead beating.
ChIP experiments were carried out using the Millipore ChIP Assay Kit (Millipore, no. 17-295) according to the manufacturer's instructions with the modifications outlined in Supporting Information.
Transformation was performed with T. virens protoplasts, essentially as previously described by Gruber et al. (1990) but with minor modifications outlined in Lopez-Mondejar et al. (2009).
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These assemblies show great promise with the minimal modification outlined in this paper they can be built better now.
PFGE was performed by using the method outlined in the BioRad Genpath Group 6 Kit (BioRad, Marnes-la-Coquette, France) with modifications outlined by Mazurek et al. (13 ) and Burki et al. (14 ).
(2009), with several modifications, as outlined in Extended Experimental Procedures.
The recombinant protein was purified as previously described [54] with some modifications outlined below.
The major steps involved in 4C aliphatic glucosinolate side chain modification are outlined in Figure 1.
To adjust these steps for use with picograms of input, we introduced modifications that are outlined in Table 1.
(2005), with the modifications for adult heads outlined in Backfisch et al.
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