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This chapter discusses theoretical approaches to assess modifications of vector mesons in the medium, as well as their experimental identification via electromagnetic probes.
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A modification of vector control principle is designed on the base of new relay control law, which is used for generation of damping oscillating modes with unlimited growth in oscillation frequency.
Improvement of the initial technologies, including modification of vector designs, targeted increase in expression strength and duration as well as improved safety characteristics, has allowed maturation of retroviral systems into efficient and promising tools that meet the technological demands of a wide variety of potential application areas.
Our studies highlight the importance of CAR protein in regulating the transduction of CNS neurons by Ad5 vectors, and suggest that modification of vector tropism to target CAR-deficient canls cachieveeve more efficient delivery of therapies to these neurons.
Finally, the complete U6 hairpin:termination signal cassette was transferred into retroviral vector L297, a slight modification of vector L087.
PCR-based one-step site-directed mutagenesis (SDM) is an efficient and rapid method to generate gene mutation(s) for study of protein structure-function relationship, identification of gene expression and modification of vector [ 1- 3].
Amplified products were ligated into a vector and transformed into cells using pCR®2.1-TOPO vector, and TOP10 chemically competent cells following the procedure outlined in the TOPO TA cloning kit (Invitrogen, Carlsbad, CA) with the following modifications: 0.8µl of vector, and an incubation time (PCR product inserted into vector) of 30 min at room temperature.
Origin and modification of vectors is shown in table 1 and figure 1.
We propose some modifications of learning vector quantization algorithms that are especially adapted to the task of incremental learning and capable of dealing with the stability-plasticity dilemma of such learning algorithms.
Based on these results it is reasonable to anticipate that new modifications of the vectors will overcome some of the immunological barriers and will further expand the applicability of adenovirus-derived vectors.
Our aim was to develop a cloning strategy that allows to generate any gene of interest (GOI) as an N- or C-terminal EYFP/ECFP-fusion without further modifications of the vectors (Fig. S1).
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