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Modifications of protocols used for extraction of DNA from soft, fresh tissues are necessary since wood properties differ from "green" material (Rachmayanti et al. 2009): Physical.
Periodic program evaluation by the project's implementation committee resulted in minor modifications of protocols which created three separate implementation phases: A (Months 1 3), B (Months 4 6), and C (Months 7 12).
Suspensions of peripheral blood mononuclear cells (PBMC) from seven crossbred Large White × Landrace pigs (6 months old) were prepared from heparinised peripheral blood samples collected under aseptic conditions during bleeding at the abattoir, using slight modifications of protocols described previously [ 12].
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Protocols 9 and 10 were two modifications of protocol 1 in which nucleotide gaps were introduced between the adjacent oligonucleotides hybridized to the target DNA (PCR product).
Structure indicators: AASTRE was associated with an increased climate of security and creation / modification of protocols (sedation/analgesia and weaning).
To isolate acidocalcisomes we used a modification of protocols described before.
This protocol is a modification of protocols described by Lanz and Schachter [ 40] and Rogers et al. [ 41, 42].
Total RNA was extracted using the following method, a modification of protocols used in the extraction of RNA from pine cones [ 34].
Adult male and female dissected brains were fixed in 4% paraformaldehyde (Electron Microscopy Sciences) for 25 minutes and labeled using a modification of protocols previously described (Certel et al., 2007).
This has motivated attempts to characterise and remedy biases in sequencing library generation, either through modification of protocols [ 2, 3] or applying bioinformatic corrections [ 4].
Protocol 8 was a modification of protocol 1 in which LCPR3L and LCPR4L oligonucleotides were used instead of LCPR3s and LCPR4s.
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