Sentence examples for modifications of oxidation from inspiring English sources
Two modifications of oxidation of methionine and carbamidomethyl of cysteine were considered.
LC-MS data was searched using Mascot 2.4 run on a local server against SwissProt allowing for trypsin cleavages and cysteine modifications of oxidation and cucurbitacin isoforms.
The putative SS biosynthetic pathway initiates the isoprenoid pathway, mediates the cyclization of oxidosqualene, and then undergoes some modifications of oxidation, glycosylation, and other secondary transformations.
Static modification of Carbamidomethylation (+57.0214 Da on C) and dynamic modifications of oxidation (+15.9949 Da on M) and phosphorylation (+79.9663 Da on STY) were employed.
HCD-MS/MS spectra were searched with fixed modification of Carbamidomethyl-Cysteine and variable modifications of oxidation (M), acetylation (protein N-term), Gln- > pyro-Glu, and acetylation (K).
Peptide tolerance was set at ± 2.0 Da with MS/MS tolerance set at ± 0.8 Da and the search set to allow for 1 missed cleavage, and allowed for fixed modifications of carbamidomethylation and variable modifications of oxidation of methionine residues.
Typical search parameters were as follows: Maximum missed cleavage setting was 1.0; the fixed modification setting was carboxyamidomethyl cysteine with a variable modification of oxidation of methionine.
Once the mass shifts were identified, the data were then re-searched with differential modifications as well as a static modification of carbamidomethylation of cysteine and variable modification of oxidation of methionine and deamination of asparagine and glutamine.
The parameters employed were: peptide tolerance ±10 ppm, fragment ion tolerance ±0.6 Da, a maximum of three missed cleavages, fixed modification of carbamidomethyl on cysteine and a variable modification of oxidation on methionine.
Peptide (parent ion) tolerance of 10 ppm, fragment ion tolerance of 1 Da, and 3 missed cleavages were allowed, and fixed modification of carbamidomethylation on Cys (157 Da) and differential modification of oxidation on Met (16 Da) were used.
The search included variable modifications of methionine oxidation and N-terminal acetylation, and fixed modification of carbamidomethyl cysteine.
