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Bacterial exotoxins catalyze specific chemical modifications of host cell components, such as the ADP-ribosylation reaction catalyzed by diphtheria toxins or the deamidation reaction catalyzed by the cytotoxic necrotizing factor (CNF) produced by Escherichia coli.
Collectively, these studies provided a paradigm of bacteria-regulated reversible modifications of host factors and illustrated remarkable virulence mechanisms that are evolved by pathogenic bacteria.
were observed and accompanied with the modifications of host macromolecules (i.e. hemocyanins and ferritins).
Pyrin inflammasome senses bacterial modifications of host Rho GTPases.
Modifications of host cell apoptosis by chlamydial infection have been intensively studied.
Well-documented modifications of host cells have already been reported [[ 1] for review].
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This exemplifies the strategy of the modification of host proteins with the aim of simplifying the production of heterologous proteins.
In contrast, modification of host cells with small polymers provided no (∼0%) protection while long chain polymers effectively prevented infection.
Engineering of novel cell lines for biotechnological processes, e.g. influenza virus vaccine production, can be achieved by the genetic modification of host cell gene expression.
Furthermore, the achieved improvements show robustness against moderate degrees of cell-to-cell variability from genetic modification of host cells via transduction.
Recent advances in manipulating cofactors include culture conditions or additive alterations, genetic modification of host pathways for increased availability of desired cofactor, changes in enzyme cofactor specificity, and introduction of novel redox partners to form effective circuits for biochemical processes and biocatalysts.
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