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Susla et al. (2007) reported that the diversity of genes can be attributed to post-transcriptional modifications of enzymes within the cell.
Also, the approach used here could be interesting as alternative or addition to the directed modifications of enzymes to alter their catalytic characteristics.
Using thermophilic enzymes as the reference point, a review of stabilization using various approaches like protein engineering, chemical modifications of enzymes and immobilization has been attempted.
Our work provides a novel approach to investigate how post-translational modifications of enzymes regulate metabolism and could have important implications for understanding the metabolic transformation of FH-deficient cancers with potential clinical applications.
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As far as the increased risk of the diseases attributed to the misfolded/unfolded/aggregated proteins are concerned, one may not deny the importance of the various methods (chemical modifications of enzyme, protein engineering techniques and additives) which maintain the protein in its three dimensional functional native form.
Activation of Caspase-2 is coordinated by complex pre- or post-translational modifications of enzyme expression or function, respectively.
As explained above, modifications of enzyme activities after 48 hours of treatment were compared with modifications of transcript levels after 24 hours of treatment.
To determine whether the observed modifications of enzyme expression were because of changes in the corresponding mRNA levels, a quantitative real-time PCR analysis was performed.
It neither involves any functionalization of supports [27] nor does it require prior modification of enzymes [41].
The most effective chemical modification of enzymes is achieved by using reagents such as activated polyethylene glycol or dinitrofluorobenzene that add hydrophobic groups.
Meanwhile, an increasing number of computational methods have been developed to assist in the modification of enzymes during the past few decades.
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