Additionally, unintended effects of the genetic modification itself cannot be excluded, as direct or indirect consequences of insertional mutagenesis, creating possible unintended metabolic effects.
Importantly, the N6 variant was therapeutically efficacious even after a 50% reduction of viral dose, thereby indicating that transgene modification itself can improve the dose efficacy of HD-Ad.
This allowed us to follow in detail the potential healtheffects and their possible origins due to the direct or indirect consequences of thegenetic modification itself in the NK603 GM maize, or due to the R herbicide formulationused on the GM maize (and not G alone), or both.
If the latter is true, our observations would indicate that only the modified form of LYL1 is subjected to degradation with the modification itself acting as a signaling event.
This so-called "cultivar" effect includes both the influence of the natural genetic background of the 2 maize lines (i.e. MON810 and the conventional counterpart) and the possible impact of the genetic modification itself.
These include work on a putative Dnd-dependent restriction-modification system, the precise nature of the DNA modification itself, the core sequence motif that targets the site-specific modification in S. lividans [22], and the increasingly well characterized novel biochemical pathway that mediates this unique biological process.
However, the evidence of selective pressures on 3Hyp site occupancy implies a functional role for the modification itself rather than being simply a coincidental mark of the hydroxylase complex acting as a chaperone during assembly and transport of collagen in the ER.
The dynamic turnover of acetylation rather than the modification itself may be key in transcriptional activation (reviewed in 55).
The authors state that the genetic modification itself could be a stress factor and may produce alterations in the seed proteome.
Other databases give access to the structure of the modification itself, but out of the protein fold context, e.g. RESID (35), BCSDB/Glycoscience (36) or GlycomeDB (37).
