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Selection of cancer gene mutations for assay design and mass spectrometric genotyping were performed as previously described[6] with modifications indicated in Methods S1.
(2010), with modifications indicated in the Extended Experimental Procedures.
FISH was performed as described Smith et al. (2004) with the TSA amplification modifications indicated in the GEISHA (Gene Expression In situ Hybridization Analysis project, http://geisha.arizona.edu).edu
In order to test whether repressed hCD2 transgenes have a histone modification pattern comparable with pericentric heterochromatin, we performed chromatin immunoprecipitation (ChIP) on chromatin prepared from sorted hCD2+ and/or hCD2- T cells from CD2 1.3B and 1.3A14 transgenics, using antibodies against various histone modifications indicated in Figure 1C.
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These modifications are indicated in Text S2 and the modified matrix is given in Text S3.
The methods and experimental protocols used to assess the effects of THF-diol and LTX-diol on the estrous cycle in female rats were as previously described (Markaverich et al. 2002a, 2002b, 2005) with slight modifications, as indicated in the text and figures.
The presence of vinculin posttranslational modifications (as indicated in this work) was also observed in the proteomic study of PBMCs collected from amyotrophic lateral sclerosis (ALS) patients; significantly higher level of vinculin nitration was observed for sporadic ALS patients compared to healthy donors [ 59].
The lysine residues carrying the GlyGly modification are indicated in bold and the superscript is the position in the protein sequence.
Thus, the widespread aggregation of endogenous metastable proteins upon loss of U34 modifications indicates an imbalance in protein homeostasis.
The analysis for the combined effect of modifications indicates an increase of 18% in the COP compared to conventional design.
The fourth painting, created in 1610-14, clearly takes the third as a model, but its many modifications indicate an artist continually rethinking even his most familiar compositions.
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