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We developed a program that generates an NCL document of a certain size, introduces modifications in that document and submits it to validation.
Purified DNA fractions (IP input, P1 and P2) were digested with MboI and amplified by ligation-mediated PCR (LM-PCR) performed as described [32] with minor modifications in that TaKaRa LA Taq (Takara Bio) was used for PCR amplification of 26 cycles.
Disadvantaged residents often have illnesses that are not primarily caused by their current residential environment and it can be unrealistic to expect modifications in that environment to influence those health problems substantially.
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We establish the transcriptional relevance of a difference in an H3K9me3+ broad modification in that it affected transcription of CACNG7.
Thus, it appears that modifications in and around the flexible loop may be key to improving fusion protein expression.
In fact, THC might be considered advantageous over curcumin as a starting material for structural modification in that the former is stable under physiological pH while the latter is not.
While Rosenwasser played an early role seeking a modification in that case, the client, Julio Salazar, only got one after a friend independently pestered the bank and sent in a complete financial package, without any help from Rosenwasser.
But a monitor of the settlement appointed by California Attorney General Kamala Harris intervened as recently as September to stop banks from foreclosing on homeowners being considered for a modification in that state — the same kind of dual-tracking that cost Davis her home.
Palmitoylation is a unique lipid-based post-translational modification in that it is reversible (Linder & Deschenes 2007; Fukata & Fukata 2010).
Faecal samples were analysed for Echinococcus spp. coproantigen with a genus-specific sandwich ELISA using the protocol originally described by Allan et al. (1992) with a modification in that the capture and conjugate antibodies were derived from different rabbit antisera.
Modifications in methodology that greatly deviate from the conventional protocol and interventions that could interfere with the results were also weighed up.
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