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During acute exercise, modifications in mononucleated blood cells occur [ 7].
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The data provided by DNA quantification also showed that aneuploid nuclei were mostly in mononucleated cells after 4 and 8 days of recovery, while during the first days of recovery the aneuploid nuclei were in bi/multinucleated cells.
DHPR and triadin are not expressed in mononucleated myoblasts (Marty et al, 2000; Zheng et al, 2002) which allowed us to distinguish between peripheral nuclei in myofibers from nuclei in mononucleated cells attached to the myofibers (Supplementary Fig S1B).
In the index case, interphase FISH showed that the translocation was present only in mononucleated cells and was absent in the characteristic multinucleated giant cells.
The activation of the Catk gene has been observed in mononucleated cells induced in vitro for osteoclast differentiation over a period of 10 days.
After von Kossa staining in mononucleated cells cultured in osteoblast differentiation medium for 14 days, mineral nodules were found deposited in these cytoplasmic cells.
As shown in Figure 5a, no Tnfsf14 expression was detected in undamaged muscle, but Tnfsf14 staining was noted in mononucleated cells on day 3 AI.
RT-PCR analysis showed different levels of Opn gene expression in mononucleated cells cultured in proliferation medium compared to osteoblast differentiation medium.
This delay (76 min ± 33 min, n = 14) is even longer than the average prometaphase period observed in mononucleated kif9 null cells.
CD68 protein, a marker expressed by multiple macrophage lineage cell types, was detected in mononucleated and multinucleated cells associated with polyethylene particles and the bone surface.
Nuclei of bi and multinucleated cells distributions were similar, with a slightly higher frequency of 2C cells than in mononucleated cells.
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