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These tested and reference mixtures were used without additional modifications in fluorescence and light extinction measurements.
Results demonstrate rapid modifications of the picosecond and nanosecond fluorescence lifetimes following short irradiation times, which are accompanied with significant modifications in fluorescence excitation-emission spectra.
Complementary analyses of samples with Fourier transform infrared spectroscopy suggest that modifications in fluorescence are correlated with photo-oxidative degradation of ABS.
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The possible enchainment of Py-MI in alternating sequences occurred randomly in polymer clusters produced modifications in the fluorescence spectra, namely the shift at the higher wavelengths due to the excimer formation.
Thus, roNaV2 allows for the assessment of chemical modification induced in fluorescence microscopy settings with high sensitivity and time resolution.
ΔF: change in fluorescence; F: background fluorescence.
Three peptides exhibited a greater degree of change in fluorescence quenching when binding the unmodified RNA versus the modified RNA; their binding could have been negatively affected by modifications (P26, P28, and P37).
It was already demonstrated that the reactive species H2O2, O2 ·−, and 1O2 did not cause any modification in the fluorescence of the probe APF [24].
In addition, results showed that JNK does not regulate autophagy induction, as no modification in the fluorescence value corresponding to DQ-BSA proteolysis was observed in cells transfected with the JNK siRNA compared with untransfected cells when cells were incubated with taxol.
Although changes in tri-H3K9 and acetyl-H3K9 amount were not statistically significant, the in situ analysis of H3K9 modifications showed an obvious increase in fluorescence intensity indicating recovery of conformational state.
This signal enhancement allows detection of protein and protein modifications in single cells comparable to fluorescence technology.
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modifications in bone
modifications in regulation
modifications in cell
modifications in yeast
modifications in control
modifications in design
modifications in size
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modifications in expression
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modifications in surface
modifications in gene
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