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One platform coordinates comparative genomics, single-molecule real-time sequencing, and mass spectrometry to discover novel epigenetic marks, such as the Dedon Lab's recent discovery of phosphorothioate and 7-deazaguanine modifications in bacterial and bacteriophage genomes in the human microbiome.
Comparison of gene expression between control (time 0) and 2 h or 6 h post-infection conditions revealed extensive modifications in bacterial gene regulation.
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Base modification in bacterial genomes is performed by two classes of DNA methyltransferases: (i) those associated with restriction-modification systems, and (ii) solitary methyltransferases that do not have a restriction enzyme counterpart.
In addition, adding cruciferous vegetables and green tea polyphenols may further bring about epigenetic modifications in the bacterial DNA or the genes that they target [ 34].
They also perform some key posttranslational modifications not possible in bacterial systems.
DGGE analysis showed that inoculants induced a significant modification in the bacterial community structure.
The pathway that introduces this modification in the bacterial EF-P is yet to be elucidated.
However, the biosynthesis pathway of this modification in the bacterial EF-P is yet to be elucidated.
However, a modification in the bacterial community structure caused by inoculation could be buffered by ecosystem resilience, which is driven by the level of diversity and interactions of the plant-soil-biota [ 11].
We report that one of these genes, Rv3371, specific to pathogenic mycobacteria, when expressed in M. smegmatis leads to modifications in colony morphotype, bacterial architecture, cell surface properties and elevated TAG levels.
The main objectives of the experiments presented here are to demonstrate that we can regulate the post-translational modification of overexpressed proteins in bacterial cells, a process we dub in-cell biochemistry, and to identify structural changes in protein interaction surfaces due to presence of PTMs using in-cell NMR spectroscopy.
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