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All primers were generated using Primer3 software (http://frodo.wi.mit.edu/primer3/) using the following modifications from the default: Product size = 50 – 150 bp; Primer Tm = 59 65°C°C; Product Tm = 80 88°C°concentrationiof of divalent cations = 3 mM, concentration of dNTPs = 0.2 mM; Table of thermodynamic parameters = SantaLucia 1998; Salt correction formula = Santalucia 1998.
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The rationale and procedure of modifications to the default FluSurge assumptions were also reported.
The shape of the curve is robust to modifications of the default parameters even when different smoothing kernels were used.
We also performed minor modifications on the default tokenizer supplied with ABNER to properly identify chemical formulas.
This strong correlation suggests that modifications of RLSB gene expression from the "default" C3 pattern to C4-type BS cell-specificity, and associated cell-type specific localization of rbcL expression, might represent one evolutionary process enabling C4 expression patterns in plants that utilize this specialized pathway for photosynthetic carbon assimilation.
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Similarly, the conflict analysis tool is a modification of the default "Intersect" tool of ArcMap.
Those modifications do not change the default behavior of the algorithm and are only forced using additional parameters.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com