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The relevant modifications for using the algorithm with the vorticity redistribution technique are also discussed.
We optimized the manufacturer's protocol with the following modifications for using small quantities of total RNA from samples.
The modifications for using the COPM with a paediatric sample suggested by Cusick et al [ 76] will be applied in this study.
The Blantyre count method for %CD4/lymphocyte determination was essentially the Blantyre count (percentage) variant described previously (4) with modifications for using pediatric blood samples and the EPICS XL-MCL flow cytometer.
Genomic DNA was extracted from dried leaves of A. oxyphylla individuals using the cetyltrimethylammonium bromide (CTAB) method (Doyle, 1990), with slight modifications for using sufficient β-mercaptoethanol as an antioxidant followed by purification using phenol-chloroform.
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GenomiPhi V2 DNA Amplification kit (GenomiPhi, GE Healthcare, Stockholm, Sweden) was used according to manufacturer's instructions on 76 samples, with modification for using 10 μl of DNA extract without sample buffer to maximize initial DNA content.
This understanding should lead to a better design of clay mineral modifications for use in polymer nanocomposites.
This approach provides insights into the CB rule and its possible modifications for use in more complicated deformations where an explicit deformation map is not available.
While the implementation of the trade-off method is performed for companies, there is nothing to prevent adapting this method, with minimal modifications, for use in other industrial applications.
It has been shown that the oxygen content of liquid tin can be adequately monitored with commercial, off-the-shelf, ultra-high-impedance electrometers and ceramic oxygen sensors, requiring only minor modifications for use in a liquid metal environment.
The Broad Institute guidelines were developed for use in a research setting, and thus require some modifications for use in a clinical setting.
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