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641(c)(1) provides that (1) the portion of any ESBT which consists of stock in one or more S corporations must be treated as a separate trust, and (2) the amount of the tax imposed by Chapter 1 on such separate trust must be determined with certain modifications detailed in Code Sec.

ISH on retinal cryosections was performed as previously described [38], [39] with the modifications detailed in [36].

After further modifications detailed in the supplemental section, the tetrahedral carbon was marked for covalent bonding to Oγ atom of Ser195.

Construction of double-strand RNA producing vectors (Fig. 1) and transformation into the A. thaliana Col-0 accession were performed as described in [34] with the modifications detailed in the Methods.

Bisulfite treatment was carried out on genomic DNA using the EZ DNA Methylation-Gold Kit® (Zymo Research, Orange, CA) according to the manufacturer's protocol with minor modifications detailed below [17].

ChIP was performed as previously described (Lim et al., 2009) with minor modifications detailed below.

Amplex Red assay kit was purchased from Invitrogen (cat. no. A12219) and used with modifications detailed below.

Real time quantitative PCR (RT-qPCR) was performed as described in Iskandar et al. [ 16] with the modifications detailed below.

Oocyte-containing follicles were scored by histomorphometric evaluation as originally described (Jones & Krohn, 1961), with slight modifications detailed previously (Tilly, 2003; Skaznik-Wikiel et al., 2007).

Potential facilitators of CKD prevention through dietary modifications detailed by our participants included individual, as well as home and community-based programs and interventions.

The ChIP and RIP protocol was based on a previously published ChIP protocol by (Glover-Cutter et al, 2008) with modifications detailed in our Supplementary Material.

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