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Subsequent TCEP addition to PABA/NO treated HL60 cells did not reverse the drug-mediated sulfhydryl modifications (data not shown) indicating an absence of disulfide bonds and perhaps, corresponding with cell surface protein thiol nitrosylation.
In contrast, the expression of CD40 and CD83 genes did not correlate with histone modifications (data not shown).
Furthermore, liquid chromatography mass spectrometry analysis of reaction products of MtDdl, d-Ala, DCS, and ATP failed to reveal MtDdl-dependent DCS modifications (data not shown).
Additionally, mutations of these three residues under neutral or positive selection are not predicted to result in changes that affect secondary or tertiary structure, or create new sites for putative posttranslational modifications (data not shown).
To further confirm the topology of phylogenetic tree, a maximum likelihood (ML) phylogenetic tree was constructed, which showed similar topology to the N-J tree with only minor modifications (data not shown).
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Mass spectrometry analyses documented that none of the purified proteins had undergone degradation or post-translational modification (data not shown).
In all cases, controls using vehicle (DMSO) or non-specific IgG antibodies did not affect the cell-induced matrix modification (data not shown).
The most potent antagonists had IC50s less than 1 nM, indicating over 1000-fold enhancement in potency by chemical modification (data not shown).
The stratified analysis revealed no effect modification (data not shown).
Peripheral neuropathy was not the main reason for regimen modification (data not shown).
This site appeared to be one of the regions most accessible to DMS modification (data not shown).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com