Sentence examples for modifications cells were from inspiring English sources

cDNA from 500,000 S2 cells was made using the cell to cDNA kit (Ambion) as per manufacturers instructions with the following modifications: cells were lysed in 100 µl lysis buffer and the RNAase inactivated by heat treatment.

For detection of PRC1 proteins and histone modifications, cells were first permeabilized for 10 minutes at RT in 0.2% Triton X-100 (TrX) in PBS.

For ChIP-seq of histone tail modifications, cells were dissolved in ChIP lysis buffer1 (10 mM Tris HCl pH 7.6, 150 mM NaCl, 1 mM EDTA, 0.3 % Igepal-630) and incubated at 4 °C for 20 min.

Preparation of metaphase chromosomes from ES cells was performed as previously described with minor modifications: cells were treated with hypotonic solution (0.56% KCl for 15 min) and after that fixated with methanol/acetic acid (3:1) solution [ 46].

Cytotoxicity assays were performed as above with the following modifications, cells were seeded at 7500 cells per well in 96-well plates and allowed to settle for 18 hours.

DNA was extracted from 2 ml cell culture (10/ml), using Qiagen, DNeasy Blood and Tissue Kit, according to the manufacture's protocol with the following modifications; cells were incubated with Lysosyme for 45 minutes prior extraction.

Following epigenetic modification, cells were washed 3× with PBS and exposed to LPS for 24 hours.

For EGFP and RsbW levels proteins were extracted from 100 mL of stationary cultures grown for 12 days at 4°C using the sonication-based method with a slight modification; cells were fixed in 1 : 1 volume of ice cold 1 : 1 (v/v) methanol/ethanol mixture for 10 min at −20°C before centrifugation.

(Preparation of Genomic DNA from Plant Tissue) of Current Protocols in Molecular Biology with following modification: cells were harvested by centrifugation at 1000 × g (3 min), then washed twice with ice-cold ddH2O, and resuspended with buffer (100 mM Tris Cl, pH 8; 100 mM EDTA, pH 8; 250 mM NaCl) containing 8 μl of proteinase K (Merck, 20 mg/ml) per 1 ml of buffer.

Cell viability was determined using the MTT assay, which was carried out as described previously [35] with slight modification, briefly, cells were seeded in 96-well plates at a density 4×104 of cells per well.

To further examine the effect of menin on histone modification, MEF cells were treated with pan-HDAC inhibitor, TricostatinA (TSA).