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Among the myriad of biologically pivotal carbohydrate modifications, attachment of β- N-acetylglucosamine (GlcNac) to Ser and Thr residues is most germane to histone biochemistry.
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A possible application of these SGSs is within the medical sector due to their enhanced solubility (compared to other graphene derivatives) and potential for surface modifications for attachment of biomolecules and drugs.
HuRBL was performed as the mRBL assay with the following modifications: cell attachment (105 cells/well) and passive sensitization with human sera (1∶10) were combined in an overnight (ON) incubation step and stimulation buffer contained 50% D2O (Sigma) for enhancement of release.
One of the most important histone modifications is attachment or removal of an acetyl group, leading either to gene activation or repression [ 5].
10- 14 Histone modifications entail attachment of various functional groups (methyl, acetyl, ubiquitin, phospho, and sumo moieties among others) to defined residues within core histones.
In both modification techniques, attachment and growth, single-walled carbon nanotubes were found to have superior electron transfer properties.
The new electrode was used as alternative of working electrodes modified with deposit materials such as activated carbon using classical modification methods (attachment or solvent evaporation methods).
Oligonucleotides were manufactured by Illumina (Invitrogen) and included a 5' amino modification for attachment to Codelink activated slides (GE Healthcare).
In these multicellular organisms, intracellular levels of UDP-GlcNAc are used as sensors of nutritional status that result in protein modification by attachment of O-GlcNAc.
DDVP exposure induced a general perturbation of fatty acid metabolism as reflected in the down-regulation of transcripts associated with the GO terms, β- oxidation, fatty acid synthesis, fatty acid modification, moiety attachment to transport, and PPAR signaling (Additional file 3, Functional analysis of differentially expressed genes).
Both modifications enhanced cell attachment and spreading.
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