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These modifications are detectable in a deep sequencing experiment, and if they are specific for miRNAs, incorporating them into a scoring system should further boost the identification of miRNAs.
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Analogous to breast cancers, this modification was detectable in 40%% of low and 46.3 % of high grade gliomas (Fig. 3a, b).
We also show that nuclear and cytosolic proteins can be easily extracted and detected by immunoblotting, and that posttranslational modifications, such as protein phosphorylation, are detectable in proteins recovered from TRIzol®-chloroform fractions stored for up to 2 years at −80°C.
Finally, acylcarnitines are detectable in normal human lavage fluid.
TIMP-2 and IGFBP7 are detectable in urine.
In contrast, no changes in global levels of either modification were detectable by western blot in bulk histones extracted from Mbd3-null lines, consistent with our hypothesis that NuRD controls H3K27 modifications only at specific target genes.
No solid state modifications and chemical interactions were detectable in the microspheres after manufacturing and during storage, suggesting their stability and use as orotransmucosal delivery systems.
Data reported in Figure 1B regarding D10 melanoma cell line as representative example indicate that proliferation in 2D cultures reached a plateau within 5 days, whereas, in contrast, no major modifications in cell numbers were detectable in MCTS within 20 days of culture.
No effect modification by years in residence per se was detectable in our study, which could be explained by the fact that most subjects had lived in their current residence for several years, thereby limiting the misclassification of long-term exposure.
Even with modifications of the PET blot technique for the detection of protein aggregates in muscle tissues [ 7], no α-synuclein aggregates were detectable in muscle fibers.
Hercynine was detectable in all the tissues studied (Fig. 5a).
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