Post-translational modification and isoform expression of OPN varies by pathological state and cell type and differentially modulates its biological activity [23] [28].
The reversible N-terminal lipid modification (palmitoylation) and isoform specific pY32 motif potentially complicate understanding Lyn's function through their latent ability to regulate activity, interactions, and subcellular localization.
DOI: http://dx.doi.org/10.7554/eLife.09336.005 10.7554/eLiFigure36.006 Figure 1 figure supplement 4. Modification at T662 and isoforms of Ypk17A both accurately report authentic in vivo phosphorylation.
Histones have distinct posttranslational modifications, variant isoforms, and dynamics.
Follistatin-like protein 1 (FSTL1) is a secreted glycoprotein with extensive glycosylation modifications and exists in two isoforms that differ in the extent of sialylation [ 1].
This study used bioinformatics and experimental approaches to conduct detailed analysis of the dysregulated miRNAs, arm selection preferences, 3' end modifications, and position shifts in isoforms of miRNAs (isomiRs) in breast cancer.
The specificity of CKα antiserum also makes it suitable for immunoprecipitation of total CKα from cell extract to study the in vivo properties of this isoform in terms of post-translational modifications and interaction with other CK isoforms [23] or proteins.
This characterization provides important basic information on expressed proteins, their isoforms and post-translational modifications, and tools for the continued investigation of the underlying molecular characteristics of hESCs.
Results have implications for future analyses of membrane proteins when maximum sequence coverage is required (e.g., mapping protein protein interactions, identification of isoforms, and post-translational modifications), and moreover, for quantitation of membrane and soluble proteins when reproducible and specific enzymatic digestion is required.
Human Glo1 exists in various isoforms; however, the nature of its modifications and their distinct functional assignment is mostly unknown.
