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FISH experiments were performed according to standard protocols with minor modifications and analyzed with an Axioplan 2 Imaging microscope (Zeiss, Sliedrecht, The Netherlands).
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From all specimens, DNA was extracted from the cortical plate (fetus) or gray matter (children, adults) using a standard procedure, with modifications [51] and analyzed by MethyLight PCR after bisulfite conversion [22], [23], [52] (for primer sequences, see Table S2).
UMAT allows users to define the progressive stiffness modification and analyze the fracturing of composite components.
This system and its modifications are simulated and analyzed thermodynamically using Aspen Plus (version 11.1).
We discuss the modifications introduced, and analyze the interaction among peers within the community, as well as the interaction of community members with the rest of the world, on the basis of the volumes exchanged.
To determine the lipid content in yeast cells, lipids were extracted, based on the method of Bligh and Dyer [ 44] with modifications and then analyzed through GC.
Furthermore, we produced new genome-wide maps for seven histone modifications, and we analyzed, for the first time in S. cerevisiae, the genome-wide profile of acetylation of lysine 8 of H4.
4– 6 The aim of this study is to identify evolutionary modification scenarios and analyze their use for engineering TCS.
To broaden the usefulness of ICSSWH systems, especially for overnight applications, numerous design modifications have been proposed and analyzed in the past.
PAHs were extracted according to Griest and Caton (1983) with modifications (Marsili et al. 2001) and analyzed by HPLC with fluorescence detection as described by Marsili et al. (2001).
In preliminary studies, we have found that our hardware and software can be employed, with minor modifications, to record and analyze song from several other species of Drosophila.
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