For both OGlcNAcylation and phosphorylation, a higher level of induced structure and a greater overall structural change were observed for threonine modification than for serine modification: of all peptides in the series with N-terminal modifications, the least α-helical peptide contained unmodified threonine while the most α-helical peptide contained dianionic phosphothreonine.
Ten dolastatin 10 analogues have been designed and synthesized with N-terminal modifications based on the known compound monomethylauristatin F (MMAF, 3).
In conclusion, we have examined the changes in Cu2+ coordination associated with N-terminal modifications that accompany the accumulation of extracellular Aβ in vivo, including isomerisation, truncation and cyclisation.
The examples described above illustrate the diversity of histones with N-terminal modifications that can be generated using protein semisynthesis.
In contrast to Aβ1 x, little has been reported about the Cu2+ coordination of Aβ with N-terminal truncations or modifications, yet the deposition of extracellular Aβ in vivo is accompanied by a large degree of amino-terminal heterogeneity.
The MKP1 interaction with N-terminal tail of H3 showed a strong preference for Ser10PLys14AcLys9Ac modifications over Ser10P alone.
Histones are highly basic globular proteins, with N-Terminal "tails" which can be heavily modified by a variety of posttranslational modifications, or histone marks [3].
This 150-kDa protein could not have been a post-translational modification, as it was detected by C-terminal-directed antibodies to APC, beyond the termination codon in SW480, in addition to it being detected with N-terminal antibodies.
Co-translational N-terminal acetylation is incompatible with N-terminal ubiquitylation (Kuo et al., 2004).
For example, addition of an N-terminal methionine, truncation of two N-terminal residues or modification the N-terminal of CCL5 with aminooxypentane generated potent antagonists for CCL5 chemokine receptors.
