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However, in addition to the prevalence of H4R3me2s in the ventricular zone, high levels of this modification were detected in the post-mitotic neurons expressing NeuN marker within the cortical plate and subplate (Fig. 2A and B).
Despite a better Prdx6 sequence coverage for the pI 6.7 compared to the pI 6.3 spot, neither parent nor daughter ions corresponding to the peptide bearing the Cys47-SO3H modification were detected in the pI 6.7 spot.
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This protein modification was detected by immunoblot and mass spectrometry (MS), and then comprehensively validated by chemical and biochemical methods.
The evidence for hydride formation during the surface modification was detected by X-ray diffraction measurements as well as by an electrochemical method.
If an entity is updated, the numeric version column is incremented by 1. Update statements always contain the version in the where-clause and if a concurrent update occurs, they do not match and the concurrent modification is detected.
O-GlcNAc modification was detected by immunoblotting using monoclonal anti O-GlcNAc antibody (Fig. 2A) suggesting that they had undergone O-GlcNAc modification.
In the analysis stratified by quartile of reported condom use (1 100%) no effect modification was detected (p = 0.63) (data not shown).
The H2A125Kme1 modification was detected both in HEK-AT1R and AngII activated HEK-AT1R cells at higher levels than in the control HEK cells.
In comparison to HCT116 cells, H3K4me1 levels were much reduced at sites A through E in HEK293 cells although this modification was detected at the promoter region (Figure 3B).
However, an obvious parental bias of H3K36me3 modification was detected.
No statistically significant effect-measure modification was detected.
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