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This protein modification was detected by immunoblot and mass spectrometry (MS), and then comprehensively validated by chemical and biochemical methods.
The evidence for hydride formation during the surface modification was detected by X-ray diffraction measurements as well as by an electrochemical method.
In the analysis stratified by quartile of reported condom use (1 100%) no effect modification was detected (p = 0.63) (data not shown).
O-GlcNAc modification was detected by immunoblotting using monoclonal anti O-GlcNAc antibody (Fig. 2A) suggesting that they had undergone O-GlcNAc modification.
The H2A125Kme1 modification was detected both in HEK-AT1R and AngII activated HEK-AT1R cells at higher levels than in the control HEK cells.
In comparison to HCT116 cells, H3K4me1 levels were much reduced at sites A through E in HEK293 cells although this modification was detected at the promoter region (Figure 3B).
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If an entity is updated, the numeric version column is incremented by 1. Update statements always contain the version in the where-clause and if a concurrent update occurs, they do not match and the concurrent modification is detected.
Despite a better Prdx6 sequence coverage for the pI 6.7 compared to the pI 6.3 spot, neither parent nor daughter ions corresponding to the peptide bearing the Cys47-SO3H modification were detected in the pI 6.7 spot.
However, in addition to the prevalence of H4R3me2s in the ventricular zone, high levels of this modification were detected in the post-mitotic neurons expressing NeuN marker within the cortical plate and subplate (Fig. 2A and B).
Indeed, high levels of tyrosine nitration are found in Lewy body aggregates of α-synuclein and the same modification is detected in the soluble protein under conditions of cellular oxidative stress.
In contrast, a different pattern of H3K4 modifications was detected in AR1 cells, which express a much higher level of Hoxa9, 41 fold higher than in AT1 cells (Fig. 1E).
More suggestions(15)
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modification was taken
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modification was accomplished
modification was shown
modification was required
modification was fixed
modification was verified
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