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To improve the specific activity and catalytic efficiency of l-LcLDH1, an NADH-dependent allosteric l-lactate dehydrogenase from L. casei, towards phenylpyruvic acid (PPA), its directed modification was conducted based on the semi-rational design.
To improve the compressibility of low-compressibility saccharides, particle modification was conducted by coating and granulating a low-compressibility saccharide with a high one to enable the production of a fast-disintegrating tablet.
When the modification was conducted in an alkaline (e.g. 0.1 M KOH) solution, in aqueous solutions under applying a potential more positive than 0.3 V vs. Ag/AgCl, or at a low modifier concentration (e.g. 20 μM), the proper 4-PySH modified surface was obtained even using 4-PySH as received, which contained a small amount of sulfide.
Modification was conducted in dry toluene.
The modification was conducted at 37°C for 1 h, each sample containing 10 mL of erythrocyte suspension of 2% hematocrit was stirred continuously.
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This modification is conducted in the assembled microfluidic cell, with minimum material and time consumption.
This modification is conducted by DNA topoisomerases and involves transient cleavage and re-ligation of the double-stranded DNA molecule.
This modification is conducted by DNA topoisomerases and involves transient cleavage of one or both strands of DNA followed by religation of the double helix (Champoux, 2001).
Re-modification was conducted by either elongating or shortening transcript ends until the average raw intensity values of the probe (not pseudomedian value) was greater than or equal to the threshold cut off.
The xylene-induced ear edema test with modifications was conducted as described previously [ 11].
Experiments on classification performance using these modifications are conducted to demonstrate their feasibility.
More suggestions(15)
alterations was conducted
changes was conducted
modification was evaluated
modification was found
modification was made
modification was observed
modification was increased
modification was assessed
modification was performed
modification was specified
modification was studied
modification was detected
modification was required
modification was fixed
modification was verified
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