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The following search parameters were selected: fixed cysteine carbamidomethylation modification, variable methionine oxidation modification, variable protein N-acetylation, and variable phosphorylation of serine, threonine and tyrosine.
missed cleavage sites: 2; carbamidomethylation at cysteine as fixed modification; variable modification: oxidized methionine; precursor mass tolerance +/− 0.05 Da; product mass tolerance +/− 0.7 Da.
This was done as follows: P = PO + ɛ σ, where P is a parameter from the Gierer Meinhardt model, PO is the original template parameter before modification, and ɛ σ is the modification variable.
Search parameters used were a precursor mass between 400 and 4500 amu, up to two missed cleavages, precursor-ion tolerance of 3 amu, accurate mass binning within PeptideProphet, semi-tryptic digestion, a static carbamidomethyl cysteine modification, variable methionine oxidation and variable phosphorylation of serine, threonine and tyrosine residues.
The search parameters used were the following: homo sapiens as the current species, a mass tolerance of +0.5 Da, an MS/MS tolerance of +0.3 Da, up to 1 missed cleavage site, fixed carboxymethyl (cysteine) modification, variable oxidation (methylation) modification, the Micromass PKL format, and the ESI Q-TOF instrument.
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The search parameters were as follows: Enzyme, Trypsin; Fixed modifications, Carbamidomethyl; Variable modifications, Oxidation; Peptide tolerance, 250 ppm; MS/MS tolerance, 0.5 Da; Max missed cleavages, 1.
Mascot search parameters were as follows: enzyme specificity trypsin, fixed modifications cysteine carbamidomethylation, variable modification methionine oxidation.
As by-products, through this modification the variable z is omitted and implicitly updated in the iterative scheme.
The search criteria were as follows: database - MSDB; organism - Drosophila; protease - Trypsin; fixed modification, Carbamidomethyl; variable modification - Oxidation (M); mass accuracy - 100 ppm and 0 missed cleavages.
Database search criteria were as follows – enzyme: trypsin, taxonomy: none, fixed modification: carbamidomethylation, variable modification: methionine oxidation, peptide mass tolerance: 120 ppm, one missed cleavage allowed.
Search parameters were as follows: enzyme; trypsin; fixed modifications: carbamidomethyl (C); variable modifications: deamidation (NQ), oxidation (M); maximum missed cleavages: 1. Deamidation (NQ) were chosen as variable modifications.
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