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The IC50 of both 2′-O-Me and 3′-deoxy ATP was decreased with the boranophosphate modification up to 16-fold.
The number of acidic sites of the modified nanocatalysts was increased by the silica modification up to 2 wt.% of SiO2 and afterward decreased.
Selected ZFN pairs were tested for the ability to induce sequence changes in a Cel1 assay and we observed frequencies of genomic modification up to 18.7% at the endogenous hTERT locus.
All samples exhibited negligible surface modification up to at least the first 5 min of the MHz sonication.
Thus, we have demonstrated that the implantation resulted in semiconductor surface modification up to the nanoscale pattern formation.
Level 2 features more extensive power supply and mainboard modification up to the change of many smd 0402 components around the main processor.
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The modifications up to 1993 were estimated to have added £24m to the cost; the most recent modifications, another £92m, making £116m altogether, or £350 per gun, to put right all the things that were wrong when production began.
The analytical solution by the two-scale perturbation analysis with modifications up to the second order is shown to be a most economical solution to give a reasonably good prediction.
Subsequent dosing modifications (up to or decreased from 100 mg/kg) were permitted based on SpO2 and observed adverse events temporally related to efaproxiral administration.
Additionally, we mixed 2′-azido, 2′-fluoro and 2′- O-methyl modifications up to a number of seven within the antisense strand.
The following search parameters were used for protein identification: (i) MS/MS mass tolerance set at 0.5 Da, (ii) peptide mass tolerance set to 20 ppm, (iii) carbamidomethylation set as a fixed modification, (iv) up to two missed cleavages were allowed and (v) methionine oxidation set as a variable modification.
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