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The resulting cell pellet was then stored at −70°C until the HK activity was assessed based on a modification to the methods by Miccoli et al (1996) as described previously (Smith et al, 2006).
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In order to accelerate the speed of the algorithm, Zhang and Wang [27] made a further modification to the method by adopting a new searching direction for the split feasibility problem.
Making a small modification to the method of Izutani and Hirasawa (1987a, b), we assume that D ij is in proportion to d ij as, (3) where the subscripts 1 and 2 stand for the main shock and the largest aftershock, respectively.
Isolated fat cells were obtained with a modification to the method of Rodbell [40].
To accurately assess the percentage of total cells labeled positive for CD-41, a modification to the method used for PGHS-1 was used.
We note that most ChIP studies do not use Spin-X columns until the wash steps, and this modification to the method was applied before the study of Waldminghaus and Skarstad [ 1], e.g. [ 21].
In this regard, the present study introduced a modification to the method of Ma et al. to recover the products from a two-gel system with a minimum agarose percentage and no primer mixture interference.
A modification to the method of Cheng et al. [ 39] was used, where the explants were co-cultured in a sterile 100 × 15 mm Petri dishes Petri dish containing a filter paper (Whatman 4) soaked with 40 μl inoculation medium at 24°C in the dark for 1 to 7 days.
This method can be easily adapted to the quantitative detection of other mutations in HIV-1 or other viruses by introducing minor modifications to the methods described.
T lymphocyte stimulation and activation was performed on individual spleens isolated from SWR and C57BL/6 mice fed either a high-fat or control diet for 6 weeks using modifications to the methods described by Trickett and Kwam [22].
Some modifications to the methods were made, e.g. the internal standard used was no longer available and adaptation to differences in equipment and instruments used.
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