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This contribution describes a simple, new approach, surface oxidation under ambient air; the method provides an efficient, high throughput, convenient, and economical way to identify double bond positions in lipids; it requires neither sample derivatization nor any special MS instrument modification to detect unsaturation sites.
Similar(58)
A commercially available enzyme immunoassay (Abbott Laboratories) was used, with some modifications to detect IgG anti-HEV in pigs.
ELISAs were carried out blind to the clinical and serological information from STH using methods published previously 10 with appropriate modifications to detect IgA.
We also investigated co-localization patterns of the two chromatin modifications to detect putative bivalent domains and the effect of MDV on such domains.
WMISH was performed essentially as described by Hinman et al. [ 18], with modifications to detect riboprobes using fluorescence as described by Denkers et al. [ 40].
To the number string searches we added modifications to detect Canadian postal codes (letter number letter number letter number) and Ontario healthcard numbers (a string of 10 numbers that may or may not be separated into groups of 4, 3 and 3 digits plus or minus one, or two letter version codes).
Second, we utilized remodeling cohorts and the modification data to detect the interactions between chromatin remodeling and modifications.
In addition, this level of performance also provides scientists confident detection and identification of modifications hard to detect at the protein level, such as deamidation.
We assessed our patients at an interval of 2 years; this period of time being suggested as a minimum for clinical trials of disease modification in OA to detect both structural and symptom change [ 29].
We also used a slight modification of DiffCoEx to detect significantly preserved modules in both male and female fly metabolomes.
Previous characterization of PHFs prepared by differential centrifugation using amino acid analysis reported a low unmodified Lys content relative to predicted tau composition, consistent with extensive Lys modification, but failed to detect meK [ 42].
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