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To validate the hormonal modulation leading the root phenotypic modification, the expression of auxin-responsive genes (AUX/IAA) of rice were assessed by reverse transcriptase quantitative PCR.
To our knowledge, this is the first demonstration that an hypomorphic variant of a transcription factor might influence CKD progression, by modulating, likely through chromatin modification, the expression of target genes, i.e. Tgfa (Fig 9).
The relative function of the two enzyme could be determined by myriad factors including those that act directly on enzyme activity (e.g., transcription, post-translational modification), the expression of auxiliary proteins (co-factors, inhibitors, proteins that present or sequester substrate) or factors that affect the relative proximity of the enzymes to their substrates.
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According to the classical ABC model and its modifications, the expression of the AP3-like gene is restricted to whorls II and III [ 7].
Up to now, in rice, the modification of the expression of more than 60 genes from diverse origins has shown beneficial effects with respect to disease resistance.
Genetic modification of the expression of these enzymes has already suggested methods to improve the disease resistance and forage quality of alfalfa.
The amiRNA technology offers a way for time-efficient modification of the expression of such genes in any variety.
Furthermore, no significant modification of the expression of the efflux pump encoding genes mexB, mexD, mexF, or mexY was detected.
Conversely, no consistent modification in the expression of such markers was observed after DMARD treatment.
This results in the passage from a transcriptionally competent to an incompetent state, which in turn determines the modification of the expression of several genes.
At the post-transcriptional modification level, the expression of the fusion transcript that was regulated by microRNAs (miRNAs) was studied.
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