As the MAbs were raised against E. coli-expressed recombinant CA XII that did not undergo posttranslational modifications, the ability of the MAbs to recognize glycosylated form of CA XII was evaluated.
The modification increases the ability of PMMA_REF to undergo plastic deformation.
From the simulation results in Section 3, we can find that both phase predistortion and spectrum modification have the ability of sidelobe suppression.
This modification alters the ability of HIF-1/HIF-2 transactivateatheireir target genes [ 9].
This modification hindered the ability of IgG1 to bind C1q and initiate complement activation, thus providing insight into the IgG1 C1q interaction.
TRF1 is poly(ADP-ribosyl)ated by tankyrase 1, and this modification blocks the ability of TRF1 to bind telomeric DNA in vitro (Smith et al, 1998).
Interestingly, while the activity of various inhibitors on a few species of bacteria has been tested, the effect of substrate modification on the ability of various UPPS proteins to recognize different substrates has not.
Here, we analyzed the pattern of IgG1 carbamylation occurring in vitro and in vivo and studied the effect of this modification on the ability of IgG1 to trigger the classical complement pathway and induce CDC and ADCC.
DMF can covalently modify proteins owing to the presence of an α/β unsaturated carbonyl group (Frycak et al., 2005; Schmidt et al., 2007), and it has been suggested that this modification reduces the ability of p65 to bind to DNA (Seidel et al., 2009).
As shown in Table 1, this modification decreases the ability of the derivatives to inhibit aromatase (fluorinated derivatives: 3, IC50=12 n m vs. 11, IC50=39 n m; chlorinated derivatives: 4, IC50=2.3 n m vs. 17, IC50=18 n m; brominated derivatives: 5, IC50=0.82 n m vs. 19, IC50=2.6 n m).
