Using a mintbody, we could detect differences in modification status among cell types in living plants.
Liu, N. et al. Probing N6-methyladenosine RNA modification status at single nucleotide resolution in mRNA and long noncoding RNA.
Moreover, iPSCs and ESCs and are extremely similar in morphology, gene and protein expression, differentiation ability, and epigenetic modification status.
Histone modification status changes between the JMJD3 knockout and parental HL-60 cells were determined across the genome using the following method.
Dekker, J. GC- and AT-rich chromatin domains differ in conformation and histone modification status and are differentially modulated by Rpd3p.
Regarding histone modification, although the acetylation levels of histone H4 have been shown to differ between dividing and differentiated cells in roots34, to our knowledge, there have been no reports on cell type-specific histone modification status.
In fact, the histone composition and modification status appears to be intimately associated with the proliferation potential of cells within developing organs.
The improved SUP MLPA and sequencing gel electrophoresis method will be useful for food and animal feed identification, bacterial detection, and verification of genetic modification status of crops.
Although H2BK120ub triggers several critical downstream histone modification pathways and changes in chromatin structure, less is known about the regulation of the ubiquitylation reaction itself, in particular with respect to the modification status of the chromatin substrate.
We evaluated the distributions of eight histone modifications across these TE-promoters, within and between the GM12878 and K562 cell lines, and related their modification status with the cell-type specific expression patterns of the genes that they regulate.
An important aspect of my research is to identify proteins in different compartments of retinal photoreceptor cells, their amount and modification status at different cell states defined by the light conditions, genotype, disease etc.
