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The histone modification state of the mir-200c cluster CpG island also shows cell type-specific differences that are closely linked to the expression state of miR-200c/141 in normal and cancer cells.
The modification state of each of these residues was analyzed by mass spectrometry, and nine out of the ten proline residues were found to be hydroxylated (Fig. 7E)- the lone exception was Pro-123.
Figure 5 shows the results of chromatin immunoprecipitations coupled to quantitative real-time PCR analysis that were used to examine the histone modification state of the miR-200c/141 CpG island in normal and cancer cells.
This convention disregards the explicit post-translational modification state of the protein or enzyme.
The upper or β-axial ligand varies, depending on the modification state of cobalamin (R-group in Fig. 1a).
Recruitment of de novo methyltransferases has been shown to depend on the modification state of the associated histones.
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RNA molecules, such as long noncoding RNAs (lncRNAs), have critical roles in regulating gene expression, chromosome architecture, and the modification states of chromatin.
Consistent with this view, many remodelling complexes contain components that recognise specific modification states of histone tails.
In addition to DNA methylation, epigenetic modification states of histones are also implicated in oncogenesis.
Chromatin connectivity destabilizes this synergistic system and introduces bistability, illustrating state switching between opposing modification states of the model gene.
DOI: http://dx.doi.org/10.7554/eLife.02996.008 10.7554/eLiFigure96.009 Figure 3 figure supplement 1. Combinatorial modification states of micronuclear H3 from conjugating Tetrahymena.
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