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A glycosyl-phosphatidylinositol (GPI) modification signal was added at the C terminus.
Both are predicted glycosylphosphatidylinositol-anchored proteins (GPI-APs) carrying a conserved GPI modification signal.
We show that GPI modification is necessary and sufficient for delivering both BG_pap and PDCB1 to Pd Moreover, the GPI modification signal from both Pd- and non-Pd GPI-APs is able to target a reporter protein to Pd, likely to plasma membrane microdomains enriched at Pd As such, the GPI modification serves as a primary Pd sorting signal in plant cells.
These proteins identified are involved in a variety of processes, including redox homeostasis, cell wall modification, signal transduction, cell defense and carbohydrate metabolism, indicating a complex regulative network in the apoplast of seedling roots under H2O2 stress.
These genes were associated with metabolism, cell-wall modification, signal transduction, transcription, and defense [ 13].
Thus, we conclude that these two tools are the most suitable for histone modification signal detection in cancer data.
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Here the signal peptides (SPs) and GPI-anchor modification signals of the six OsRUSs were predicted by SignalP 4.0 (http://www.cbs.dtu.dk/services/SignalP/) and BigPI (http://mendel.imp.ac.at/gpi/plant_server.html), respectively.
In addition, 21 SEGs in the ∆fliC-treated library were identified to be involved in secondary metabolism, RNA transcription, protein degradation and modification, signaling and transport (Figure 7 and Additional file 6: Table S7).
We did not find obvious H3K27me3 modification signals at the SRC3 promoter.
This explains why Bsm1 and Taq1 show similar effect modification signals.
Histone modification signals were ∼3.5 13.6 times as intense as those seen along chromosome 12.
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