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Additionally, when the PEG modification rates are same, the bioactivity of PEGylated TNF-α decreased with an increase in the molecular size of the attached PEG.
Quantified locus modification rates are indicated below relevant lanes.
Quantified locus modification rates are indicated below each gel.
Thus, modification rates are obtained from the slope of a logarithmic graph, and each rate relies on multiple measurements.
Note that any terminal residue matching genomic sequence was considered as templated, so the modification rates are underestimated.
Modification rates are determined by plotting the extent of side chain modification, quantified by mass spectrometry, versus X-ray exposure time.
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Particularly high initial antibiotic treatment modification rates were observed in the UK (37.7%; n = 43/114) and in Belgium (35.6%; n = 68/191), while low initial antibiotic treatment modification rates were observed in France (15.6%; n = 57/366) and Greece (21.9%; n = 47/215).
For fragments containing Met residues, the modification rates were determined from doubly modified fragments, because peaks for singly modified fragments containing nonoxidized Met were not apparent.
Residue-specific modification rates were determined by integration of peak areas and plotting those areas as a function of multiple exposure times.
Furthermore, we found that the reduced modification rates were mainly owing to the decrease of A modification, implying that HBV infection can repress the mechanisms responsible for the A modification without affecting the other modifications.
Because it is difficult to be certain that any preparation of Aβ40 is fully unstructured, modification rates were also measured for Aβ40 that had been digested with pepsin prior to irradiation to provide a sample closely representing an unstructured state.
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