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Experimental protection factors were calculated by dividing the modification rate for predigested Aβ40 peptides (presumed to be mostly if not fully structure free) by the modification rates for fibrillar and LMW Aβ40, thus normalizing the effects of residue-specific reactivity to provide a pure measure of structure.
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3) The indel modification rates for the gels shown should be quantified.
(E ) Total modification rates for strand scission or reverse transcription stops calculated from CE or sequencing data.
Parameters b and a denote the decay and modification rates for mRNA respectively; while d and e are used for the protein.
This "predigested" Aβ40 yielded baseline modification rates for side chains in the context of minimally structured segments while preserving the sequence context of the residues of interest.
We have specified the time points in the legends of Figure 1, 2, 4, and 5. 3) The indel modification rates for the gels shown should be quantified.
In conclusion, this study provides 'real-world' data showing significant differences in treatment modification rates for exenatide BID and insulin glargine, indicating potential for longer treatment durability for patients initiating exenatide BID.
Therefore, we repeated the analysis with new LP preps and carried out the initial analysis of CCR5-directed disruption under experimental conditions providing almost similar modification rates for LP- and plasmid-directed ZFN delivery (slightly higher efficiency with protein transduction; 8 % vs. 7%%).
Protection factors normalize the modification rate data for differences in side chain reactivity, and yield a measure of structurally important differences in solvent accessibility of the Aβ40 states at the single-residue level.
However, due to the low abundance of the singly modified 419A-450A peptide when CN was modified using a low molar excess of DMBNHS, and subsequently the low abundance of the ETD-MS/MS product ions, the DMBNHS modification rate constants for these residues were only calculated from the 20-, 50-, and 100-fold DMBNHS molar excess reaction conditions.
In recent studies, zinc finger nuclease (ZFN -mediated genomic modification rates of 3% - 100% for specific genes have been reported in ZFN -mediatedabidopsis, and rat [ 4- 16].
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