Sentence examples for modification oxidation of from inspiring English sources
In contrast to lignin modification, oxidation of cellulose impaired enzymatic hydrolysis.
ROS damages DNA by causing strand breakage, base modification, oxidation of deoxyribose, and DNA-protein cross-links [ 114].
As fixed modification, carbamidomethylation of cysteine residues was used, and as variable modification oxidation of methionine residues was selected.
Parent and fragment mass tolerances were, respectively, set to 20 p.p.m. and 0.3 Da, partial modification (oxidation) of methionine residues being allowed.
Search settings allowed one missed cleavage with the trypsin enzyme selected, one fixed modification (carboxymethylated cysteine) and one variable modification (oxidation of methionine).
one missed cleavage site carbamidomethylation of cysteines as fixed modification oxidation of methionine, pGlu for N-terminal Gln as variable modifications 100 p.p.m. tolerance for MS- and 0.2 Da-tolerance for MS/MS-signals only doubly and triply charged ions.
During biosynthesis of amphotericin, the macrolactone core undergoes three modifications: oxidation of a methyl branch to a carboxyl group, mycosaminylation, and hydroxylation.
The MASCOT search engine (version 2.4) was used for peptide sequence searching against the UniProt database, setting the following restrictions: Homo sapiens taxonomy (Human), parent ion tolerance ±20 ppm, MS/MS error tolerance ±0.1 Da, alkylation of cysteine residues (fixed modifications), oxidation of methionine (variable modifications), and two potentially missed trypsin cleavages.
Precursor mass accuracy (10 ppm), MSMS mass accuracy (0.6 Da), enzyme (Trypsin, missed cleavages = 1), Fixed modifications (Carbamidomethylation of cysteine), Variable modifications (Oxidation of methionine, phosphorylation of S, T, Y), minimum ion score = 20.
The database searches were constrained by allowing for trypsin cleavage (with up to two missed cleavage sites), fixed modifications (carbamidomethylation of Cys residues) and variable modifications (oxidation of Met residues and N-terminal pyroglutamate formation).
The following parameters were used for searches using SEQUEST v.3.3.1 SP1: enzyme, trypsin; fixed modifications, carboxymethyl cysteine; variable modifications, oxidation of methionine; peptide tolerance, ±1.50 amu; fragment ion tolerance, ±0.5 amu; and number of missed cleavage sites, 1.
