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All experiments were performed with chemicals and solvents of reagent grade and were used without further modification or purification.
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Synthesized difunctional iniferter was very stable under basic condition and allowed the sequential reactions without chain-end modification and purification steps.
Protein Expression, Modification, and Purification The 7c12 monobody as isolated from a phage-display library was not sufficiently soluble for crystallization trials.
Although the expressions and purifications were successful, none of these studies reported production or purification yields.
No modification or extra equipment is needed.
In contrast, the sludge oilfield wastewater remained visibly unchanged after treatment while utilizing Fe3O4 nanoparticles without surface modification as the purification agent (Fig. 3c).
Thermal stability experiments with the MBP-PptT fusion using a dye-binding thermal stability assay led to modifications of purification buffer conditions to promote higher thermal stability.
Although cultured mammalian cells are preferred for producing functional mammalian proteins with appropriate post-translational modifications, purification of recombinant proteins is frequently hampered by low expression.
Laccase POXC [ 20] was purified from P. ostreatus with slight modifications in the purification protocol.
The conjugation of glycan with PNPA was carried out using the common reductive amination procedure for free glycan labeling with modifications on sample purification.
However, existing methodologies rely on sequence modifications and several purification rounds involving precipitation and multiple chromatographic steps to obtain CA from host' contaminating proteins.
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