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Building from recent achievements in the site-specific modification of virus for covalent surface tethering, we show in this work that stable 2D virus patterns can be generated in custom geometries over large area glass surfaces to yield templates of biological, biochemical, and inorganic materials in high density.
It has been shown that modification of virus capsids by PEGylation can significantly dampen the T-cell mediated immune response against the virus and stimulate the antibody response against a secreted antigen [26], [27], [30], [54].
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Genetic modification of viruses can confound the usual DNA sequencing methods used to identify the virus and make it even more difficult to track down the source.
Whereas genetic modification of viruses to evade the immune system is a daunting challenge and could yield hazardous variants, physical coating provides a simple "cover-all" that is not inherited by progeny viruses.
These results provide some insight about the degree of T and B cell mediated immunity necessary for protection against Ebola virus and suggest that modification of the virus capsid can influence the type of immune response elicited by an Ad5-based vaccine.
In the spleen, Ad genome levels were affected more modestly by IR800 modification of the virus.
Incorporating an RhPV IRES element into alphavirus vectors offers an alternative solution that does not require the expression of fusion proteins or modification of the virus structural proteins.
Modification of the virus induced a slight increase of IgA in the presence of pre-existing immunity with respect to that seen in naïve animals given unmodified virus (Vaccine, Figure 6D) and those with pre-existing immunity (Vaccine+PEI).
Modification of herpes virus particles with envelope penetrating lipophilic radioactive labels has been demonstrated and was used for biodistribution studies [ 16, 42].
In the present study we have modified fowl plaque virus (A/FPV/Rostock/34 (H7 N1)) by introducing the NP cleavage site and removing the M2 cleavage site and have analyzed the effects of these modifications on virus growth and pathogenicity.
The phenomenon of host specific restriction and modification of bacterial viruses stemmed from endonucleases within the cells that destroy foreign DNA molecules.
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