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Considering the high yield, simplified purification procedure, and high-quality of GO, the modification of using graphite flakes with small sizes is an important step towards the mass-production of GO at industrial scale.
For these reasons, a classic Ilizarov fixator using tension wires in the proximal femoral segment is not popular [12, 14] leading to, in 1986, Italian surgeons Catagni and Cattaneo introducing the hybrid modification of using half-pins but maintaining all wires in the distal femur [11, 12].
Evans followed the Baxter process, with the modification of using relief wood blocks only.
The miRNA precursors and inhibitors as well as the positive and the negative control miRNAs were transfected at a final concentration of 25 nM using DharmaFECT 1 transfection reagent (ThermoSCIENTIFIC- Dharmacon) according to the manufacturer's instructions with the modification of using 8 µl of reagent instead of 6.
The siZEB1 was dissolved in 1× siRNA buffer (ThermoSCIENTIFIC- Dharmacon, B-002000-UB) at a final concentration of 20 µM and was transfected at a final concentration of 25 nM using DharmaFECT 1 transfection reagent (ThermoSCIENTIFIC- Dharmacon) according to the manufacturer's instructions with the modification of using 8 µl of reagent instead of 6.
3′ RACE was performed as for gene expression cDNA synthesis with the modification of using the 3′ AP primer to create a 3′ adapter.
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Intrinsic pyrazinamide resistance (9 ) of M. bovis required modification of use of empiric antimicrobial drugs.
Dorran, D., Lawlor, R. & Coyle, E. High quality time-scale modification of speech using a peak alignment overlap-add algorithm (PAOLA).
This chapter outlines several methods for analysis of the processing and modification of RNA using microarrays.
The graft modification of cellulose using living/controlled polymerization methods was summarized.
Modification of metabolism using genetic engineering approaches has recently focussed on altering the biosynthesis of glutamine, polyamines, glutathione, and lignin.
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