HIV MAbs 924 and 7B2 were conjugated separately to PAC and RAC by using a modification of the protocol described elsewhere23, 24.
In order to confirm internalization of ITs to the cells, we used a modification of the protocol described elsewhere45,46,47.
The need to overcome significant community reluctance to engage in discussion of mental health resulted in significant modification of the protocol.
The QFS method is a modification of the protocol that was first described by McDonald and Webb [36].
To induce brain death, a slight modification of the protocol described by Purins et al. was used [15].
Cell growth was measured by modification of the protocol of LDH Cytotoxicity Assay (WST-8 mix reagents, BioCat, Mountain View, CA, USA).
In this study, it was hypothesized that a modification of the protocol to intermittent pacing would permit time for partial recovery of myocardial energetics leading to improved cardiac function and development of hypertrophy.
Once a desired level (>80%) of free spores were observed via phase contrast microscopy, spores were purified using a modification of the protocol of Zhao et al. (Zhao et al. 2008).
The only modification of the protocol is that BL21 competent cell was used to transform the recombination PCR product and all transformation products were cultured on Amp-LB plate containing 2 mM IPTG.
The assay was carried using a modification of the protocol described by Maguire et al. [10].
SSH was performed using a modification of the protocol given by Diatchenko et al. [17].
