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This is a modification of the method-II (Barbiero 2010).
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Collagen type-II was extracted from fetal bovine cartilage using a modification of the methods employed by Miller et al. [44].
The native molecular weight of MIF-II was estimated by using a column of Sephacryl S-300 (1×60 cm) by modification of the method of Wollny et al. [34].
The DNA isolation method was a modification of the method used in tobacco [38].
Deglycosylation of DBP was done using a modification of the method of Yamamoto et al [17].
Determination of cellular GSH content was performed by a modification of the method of [37].
Preparation of beads was done using a modification of the method of Yamamoto et al [17].
PIPLC digestion was performed by a modification of the method of Gibney et al. [94].
For primary screening, we followed a modification of the method of Hang et al [6].
Immunoblotting was performed using a modification of the method of Besch et al [26].
The method used for total protein staining using Copper [II] Phthalocyanine 3,4,4",4"' Tetra Sulfonic acid tetrasodium salt (CPTS) was a modification of the method described by Bickar and Reid (7).
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CEO of Professional Science Editing for Scientists @ prosciediting.com