Sentence examples for modification of protocol from inspiring English sources

Protocol 8 was a modification of protocol 1 in which LCPR3L and LCPR4L oligonucleotides were used instead of LCPR3s and LCPR4s.

Protocol 7 was a modification of protocol 1 in which the Taq DNA ligase and its respective buffer (10X) were replaced by 10 units of PFU ligase (Stratagene, La Jolla, CA, USA) and 0.525 µl PFU buffer (10X).

DNA was extracted from i) one single individual per inbred line (a male in the case of D. pseudoobscura and D. miranda); and ii) ten individuals per isochromosomal line, using either a modification of protocol 48 in Ashburner [33] or the PUREGENE DNA Purification kit (Gentra Systems , Inc.

Individual samples were soaked in 300  μl of butanol and heated at 95 °C for 5 min to remove lipids (modification of protocol for this study).

The DPPH (1, 1-diphenyl-2-picryl-hydrazyl) bioassay was performed according to the slight modification of protocol of Said et al. [ 12].

We then define recall and precision as follows: Evaluation protocol 2B: this is a modification of protocol 2A to check the agreement between TSR predictions and gene annotation.

Structure indicators: AASTRE was associated with an increased climate of security and creation / modification of protocols (sedation/analgesia and weaning).

To isolate acidocalcisomes we used a modification of protocols described before.

This protocol is a modification of protocols described by Lanz and Schachter [ 40] and Rogers et al. [ 41, 42].

Total RNA was extracted using the following method, a modification of protocols used in the extraction of RNA from pine cones [ 34].

Adult male and female dissected brains were fixed in 4% paraformaldehyde (Electron Microscopy Sciences) for 25 minutes and labeled using a modification of protocols previously described (Certel et al., 2007).