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ULTI (Urothelial Large T, Inducible) cells were isolated from these mice using a modification of procedures described by Kreft et al [16], [17].
Islet isolation was accomplished by collagenase digestion and differential centrifugation through Ficoll gradients using a modification of procedures described previously for rat islets [34].
Cells were purified from breast tissue from reduction mammoplasties using a modification of procedures described previously (Gomm et al, 1995; Slade et al, 1999).
To localize the expression of BRP-39 and IL-13Rα2, double-label IHC was undertaken with a modification of procedures described previously by our laboratory (Lee et al., 2009).
The continued modification of procedures also demands constant public engagement efforts to avoid dissemination of conflicting messages and to ensure that information released is up to date and presented at a level that can be adequately understood.
Protein lysates were prepared from cultured cells or whole lungs using RIPA lysis buffers and subjected to immunoblotting using a modification of procedures described previously by our laboratory (Lee et al., 2009).
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Let me state right away that I think that modifications of procedures which are working fairly well should be contemplated with caution, since a procedure like the NRMP is a vast improvement over the chaotic conditions which preceded it, and since we have seen that unstable centralized procedures are themselves prone to unravel.
Medical care personnel also need regular updates on modifications of procedures and ongoing reemphasis of infection control practices.
ChIPs of histone modifications on native or crosslinked chromatin were performed with minor modifications of procedures described previously (Cuthbert et al., 2004; Daujat et al., 2009).
The two most common transvaginal methods for placement of a cervical stitch are modifications of procedures developed by Shirodkar and McDonald [ 94, 95].
Furthermore, the proposed list of criteria can be transferred to other cancer screening procedures after suitable modification of the procedure-specific criteria.
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