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The pattern of effects on force and actin filament velocity suggests a model where S-NO modification of muscle myosin alters the attached time and duty cycle of myosin.
Bisulfite modification of muscle genomic DNA extracted from stage 2 larvae was performed using the EpiTect Bisulfite Kit (Qiagen), according to the manufacturers' instructions.
Exercise treatments can alter sensory input from the periphery by modification of muscle control (ie, muscle coordination and strength) and through improved proprioception to enhance control of the affected joint, thus reducing nociceptor discharge and enhancing normal sensory input.
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Expression and immune modulation are primarily regulated by promoter/enhancer elements, and modification of muscle-restricted enhancer plus promoter elements enables strong expression in muscle but not in immune effector cells.
We attempted to detect oxidative modifications of muscle proteins by measuring level of HNE.
Therefore, the importance of post-translational modifications of muscle cell proteins for normal muscle function, in particular α-DG, has become increasingly clear.
Notably, a recent study demonstrated that subtle in vivo modifications of muscle stiffness, by means of fibroblast-mediated collagen VI deposition, are able to affect muscle satellite cell self-renewal and maintenance [77].
Oxidative stress-induced modifications of muscle proteins can result in unfolding of the targeted protein domains [ 45], leading to an increased exposure of hydrophobic residues that are prone to activate the proteasome [ 45, 46].
SEMG, which is a non invasive technique and needle free, from this point of view might be useful in assessing the modifications of muscle characteristics in FM patients and it might be an instrument to support diagnosis, to individuate target therapies and to evaluate disease evolution and improvement during a period of time.
Nevertheless, many recent load adaptation studies have demonstrated that sufficient practice in the presence of external dynamic loads restores the original kinematic and spatial aspects of the movements (Flash and Gurevich 1997; Shadmehr and Mussa-Ivaldi 1994), even though this may require significant modifications of muscle activation patterns (Padoa-Schioppa et al. 2004).
For example, modification of striated muscle proteins by phosphorylation and proteolytic cleavage are readily observed using proteomic technologies while these changes would not be identified using genomic technology.
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